To evolve normality, the proportion analysis was indeed turned just before analyses

A standardized, cumulative list regarding cross-physical fitness are calculated per mix of maternal tree and you can pollen donor, considering mature fresh fruit lay, vegetables germination, and you will survivorship and development of seedlings. Each varieties, mixed-design study regarding difference was applied to evaluate the effects applications de rencontres coréennes away from crossing procedures (repaired effect; which have maternal forest provided since the a random perception) into percentage of give-pollinated plant life setting mature good fresh fruit, prices off seed germination and you will seedling survivorship, seedling proportions at the 1 yr, and you may collective physical fitness. Multiple patterns have been tested using ANOVA: (a) as well as the providers, (b) leaving out unbalanced services, allowing testing regarding interaction conditions, (c) without selfing procedures (as the maternal woods have been largely otherwise totally notice-incompatible), and you will (d) collection all of the within-Sinharaja outcrossing providers to check the effect regarding within this- versus. between-tree crossing. The end result out of crossing range on every parameter was next checked using linear or quadratic regression data, with regards to the form of the connection. Finally, for every single maternal forest, the consequences out of nearby-neighbor and enough time-point mating was projected thanks to indicator away from biparental inbreeding despair and you may outbreeding depression, correspondingly, considering cumulative fitness opinions.

Syzygium rubicundum

Fruit abortion was heavy for all trees, resulting in low fruit set (range across treatments: 2.0–9.7%; Fig. 2a). The timing of abortion was not discernable across treatments. Self-compatibility was low, but variable, across maternal trees (Fig. 2a). Flowers used for tests of apomixis (N = 360) and autogamy (N = 582) failed to set fruit. All analyses of variance in fruit set revealed a highly significant treatment effect and significant maternal tree effect, but no significant interaction between treatment and maternal tree (Tables 2A and 3A). For all three trees, the percentage of experimental flowers setting mature fruit showed a consistent increase with crossing distance, followed by a severe decline in fruit set with the distant between-forest treatment (Fig. 2a). The relationship between crossing distance and fruit set was nearly identical for the three maternal trees and significant with or without the self-pollinated treatment included in the model (quadratic regression model: arcsine square-root [fruit set] = crossing distance [km] + crossing distance 2 ; results without self-pollinated treatment: Fdos,57 = 8.25, P < 0.0007, R 2 = 0.47). Peak mean fruit set occurred at a crossing distance of 1–2 km (distant within-forest treatment) and was 1.7–4.7 times greater than mean fruit set rates for other hand-pollination treatments, averaged across maternal trees. Mean fruit set rate for the distant within-forest treatment was significantly greater than those for all treatments except distant-neighbor and open-pollinated, but consistently exceeded fruit set of open-pollinated flowers (Fig. 2a).

Shorea cordifolia

Fruit set was also low for Sh. cordifolia (range across treatments: 0–5.3%; Fig. 2b). Again, the timing of fruit abortion was not discernable among treatments. Selfed and distant between-forest treatments resulted in 0% and <1% fruit set, respectively. Fruit set from the intermediate-distance cross-pollinations varied across maternal trees, but with one exception (nearest-neighbor treatment at Tree number 1) indicated optimal fruit set at an outcrossing range of ?2 km (distant neighbor treatment; Fig. 2b). All analyses of variance in fruit set revealed a highly significant treatment effect, but no maternal tree effect (Tables 2B and 3B). The relationship between crossing distance and fruit set was significant only when the selfed treatment was excluded (quadratic regression model: arcsin square root [fruit set] = crossing distance [km] + crossing distance 2 ; F2,57 = 5.71, P < 0.006, R 2 = 0.41). At each maternal tree, fruit set rate for open-pollinated flowers was greater than that for all hand-cross treatments, suggesting that some aspect of the hand-pollination procedure (e.g., flower handling, bagging) caused reduced fruit set in Sh. cordifolia.

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